Mutagenesis, Site-Directed Concept

subject area of

• A New Function of Human HtrA2 as an Amyloid-β Oligomerization Inhibitor  Journal Articles
• A Thiolate Anion Buried within the Hydrocarbon Ruler Perturbs PagP Lipid Acyl Chain Selection  Journal Articles
• A covalently linked recombinant albumin dimer is more rapidly cleared in vivo than are wild-type and mutant C34A albumin  Journal Articles
• A glycosyl hydrolase activity of mammalian reovirus σ1 protein can contribute to viral infection through a mucus layer 1 1Edited by M. Yaniv  Journal Articles
• A p56 Ligand Serves as a Coactivator of an Orphan Nuclear Hormone Receptor  Journal Articles
• Active site labeling of the gentamicin resistance enzyme AAC(6')-APH(2") by the lipid kinase inhibitor wortmannin  Journal Articles
• Aerial development in Streptomyces coelicolor requires sortase activity  Journal Articles
• Altering Heparin Cofactor II at VAL439 (P6) either Impairs Inhibition of Thrombin or Confers Elastase Resistance  Journal Articles
• Amino Acid Substitutions in the Herpes Simplex Virus Transactivator VP16 Uncouple Direct Protein-Protein Interaction and DNA Binding from Complex Assembly and Transactivation  Journal Articles
• Amino acid substitutions of the P2 residue of human antithrombin that either enhance or impair function  Journal Articles
• Amplification of TGFβ Induced ITGB6 Gene Transcription May Promote Pulmonary Fibrosis  Journal Articles
• BCL11A represses HBG transcription in K562 cells  Journal Articles
• BRAF status modulates Interelukin-8 expression through a CHOP-dependent mechanism in colorectal cancer  Journal Articles
• Bax Homodimerization Is Not Required for Bax to Accelerate Chemotherapy-induced Cell Death  Journal Articles
• Bcl-2 changes conformation to inhibit Bax oligomerization  Journal Articles
• Binding Site Determinants for the LysR-Type Transcriptional Regulator PcaQ in the Legume Endosymbiont Sinorhizobium meliloti  Journal Articles
• Catalytic Mechanism of Fungal Homoserine Transacetylase  Journal Articles
• Characterization of a cryptic plasmid from Pseudomonas sp. and utilization of its temperature-sensitive derivatives for genetic manipulation  Journal Articles
• Characterization of mice harboring a variant of EPCR with impaired ability to bind protein C: novel role of EPCR in hematopoiesis  Journal Articles
• Characterization of yeast homoserine dehydrogenase, an antifungal target: the invariant histidine 309 is important for enzyme integrity  Journal Articles
• Cleavage-dependent Ligation by the FLP Recombinase  Journal Articles
• Common β‐lactamases inhibit bacterial biofilm formation  Journal Articles
• Computational Study of the Binding Modes of Caffeine to the Adenosine A_2AReceptor  Journal Articles
• Conserved, unstructured regions in Pseudomonas aeruginosa PilO are important for type IVa pilus function  Journal Articles
• Control of Centromere Localization of the MEI-S332 Cohesion Protection Protein  Journal Articles
• Control of Motoneuron Survival by Angiogenin  Journal Articles
• Crystal Structure of the Passenger Domain of the Escherichia coli Autotransporter EspP  Journal Articles
• Cyclin B-dependent kinase 1 regulates human TRF1 to modulate the resolution of sister telomeres  Journal Articles
• Cyclin-dependent Kinase 5 (Cdk5) Activation Domain of Neuronal Cdk5 Activator  Journal Articles
• Cytokine-Induced Hepatic Apoptosis Is Dependent on FGL2/Fibroleukin: The Role of Sp1/Sp3 and STAT1/PU.1 CompositecisElements  Journal Articles
• Definition of a Consensus Transportin-specific Nucleocytoplasmic Transport Signal  Journal Articles
• Determination of the origin-specific DNA-binding domain of polyomavirus large T antigen  Journal Articles
• Different binding sites for the neuropeptide Y Y1 antagonists 1229U91 and J-104870 on human Y1 receptors  Journal Articles
• Distinct lipid effects on tBid and Bim activation of membrane permeabilization by pro-apoptotic Bax  Journal Articles
• Distinct surfaces on Cdc5/PLK Polo-box domain orchestrate combinatorial substrate recognition during cell division  Journal Articles
• Effects of metal binding affinity on the chemical and thermal stability of site-directed mutants of rat oncomodulin  Journal Articles
• Eliminating Competition: Characterizing and Eliminating Competitive Binding at Separate Sites between DAHP Synthase’s Essential Metal Ion and the Inhibitor DAHP Oxime  Journal Articles
• Evidence That the Epidermal Growth Factor Receptor on Host Cells Confers Reovirus Infection Efficiency  Journal Articles
• Evidence that Herpes Simplex Virus VP16 Is Required for Viral Egress Downstream of the Initial Envelopment Event  Journal Articles
• Expression in a cell-free system of normal and variant forms of human antithrombin III. Ability to bind heparin and react with alpha-thrombin  Journal Articles
• Factor VII Deficiency Caused by a Structural Variant N57D of the First Epidermal Growth Factor Domain  Journal Articles
• Function and Redundancy of the Chaplin Cell Surface Proteins in Aerial Hypha Formation, Rodlet Assembly, and Viability in Streptomyces coelicolor  Journal Articles
• Functional Analysis of Disulfide Linkages Clustered within the Amino Terminus of Human Apolipoprotein B  Journal Articles
• Functional role of conserved residues in the characteristic secretion NTPase motifs of the Pseudomonas aeruginosa type IV pilus motor proteins PilB, PilT and PilU  Journal Articles
• Functionally important amino acids in Saccharomyces cerevisiae aspartate kinase  Journal Articles
• Gene transcription of fgl2 in endothelial cells is controlled by Ets‐1 and Oct‐1 and requires the presence of both Sp1 and Sp3  Journal Articles
• Heparin cofactor II is more sensitive than antithrombin to secretory impairment arising from mutations introduced into its carboxy-terminal region  Journal Articles
• Homology Model of Dihydropyridine Receptor: Implications for L-type Ca2+ Channel Modulation by Agonists and Antagonists  Journal Articles
• How Batrachotoxin Modifies the Sodium Channel Permeation Pathway: Computer Modeling and Site-Directed Mutagenesis  Journal Articles
• HypF, a Carbamoyl Phosphate-converting Enzyme Involved in [NiFe] Hydrogenase Maturation  Journal Articles
• IMPACT OF MUTATIONS AT THE P4 AND P5 POSITIONS ON THE REACTION OF ANTITHROMBIN WITH THROMBIN AND ELASTASE  Journal Articles
• Identification and analysis of all components of a gel retardation assay by combination with immunoblotting.  Journal Articles
• Identification of a cluster of residues in transmembrane segment 6 of domain III of the cockroach sodium channel essential for the action of pyrethroid insecticides  Journal Articles
• Identification of the Catalytic Residues of AroA (Enolpyruvylshikimate 3-Phosphate Synthase) Using Partitioning Analysis  Journal Articles
• Inscribing the Perimeter of the PagP Hydrocarbon Ruler by Site-Specific Chemical Alkylation  Journal Articles
• Intracellular events determine the fate of antithrombin Utah  Journal Articles
• Involvement of a calpain-like protease in the processing of the murine interleukin 1 alpha precursor  Journal Articles
• Kinetic Analysis of a Unique Direct Prothrombinase, fgl2, and Identification of a Serine Residue Critical for the Prothrombinase Activity  Journal Articles
• Mechanism of allosteric inhibition in the Plasmodium falciparum cGMP-dependent protein kinase  Conferences
• Mechanism of aminoglycoside 3'-phosphotransferase type IIIa: His188 is not a phosphate-accepting residue  Journal Articles
• Mechanism of aminoglycoside antibiotic kinase APH(3')-IIIa: role of the nucleotide positioning loop  Journal Articles
• Modification of Pseudomonas aeruginosa Pa5196 Type IV Pilins at Multiple Sites with d -Ara f by a Novel GT-C Family Arabinosyltransferase, TfpW  Journal Articles
• Modularity of protein function: Chimeric interleukin 1.beta.s containing specific protease inhibitor loops retain function of both molecules  Journal Articles
• Modulation of Clearance of Recombinant Serum Albumin by Either Glycosylation or Truncation  Journal Articles
• Molecular Mechanism for Lateral Lipid Diffusion between the Outer Membrane External Leaflet and a β-Barrel Hydrocarbon Ruler  Journal Articles
• Molecular Mechanism of the Enterococcal Aminoglycoside 6‘-N-Acetyltransferase‘:  Role of GNAT-Conserved Residues in the Chemistry of Antibiotic Inactivation  Journal Articles
• Molecular evidence for dual pyrethroid-receptor sites on a mosquito sodium channel  Journal Articles
• Molecular mechanism of VanHst, an alpha-ketoacid dehydrogenase required for glycopeptide antibiotic resistance from a glycopeptide producing organism  Journal Articles
• Molecular mechanism of aminoglycoside antibiotic kinase APH(3')-IIIa: roles of conserved active site residues  Journal Articles
• Mutagenesis and peptide analysis of the DRY motif in the α2A adrenergic receptor: evidence for alternate mechanisms in G protein-coupled receptors  Journal Articles
• Mutation of Any Site of N-Linked Glycosylation Accelerates the In Vivo Clearance of Recombinant Rabbit Antithrombin  Journal Articles
• Mutational analysis of state-dependent contacts in the pore module of eukaryotic sodium channels  Journal Articles
• Mutations of AKT3 are associated with a wide spectrum of developmental disorders including extreme megalencephaly  Journal Articles
• NMR Structures Reveal How Oxidation Inactivates Thrombomodulin  Journal Articles
• NleG Type 3 Effectors from Enterohaemorrhagic Escherichia coli Are U-Box E3 Ubiquitin Ligases  Journal Articles
• Nuclear import of the BTB/POZ transcriptional regulator Kaiso  Journal Articles
• Pentavalent Ions Dependency Is a Conserved Property of Adenosine Kinase from Diverse Sources:  Identification of a Novel Motif Implicated in Phosphate and Magnesium Ion Binding and Substrate Inhibition  Journal Articles
• Pervasive Cryptic Epistasis in Molecular Evolution  Journal Articles
• PhoU Allows Rapid Adaptation to High Phosphate Concentrations by Modulating PstSCAB Transport Rate in Sinorhizobium meliloti  Journal Articles
• Phosphorylation at the carboxy terminus of the 55-kilodalton adenovirus type 5 E1B protein regulates transforming activity  Journal Articles
• PilF Is an Outer Membrane Lipoprotein Required for Multimerization and Localization of thePseudomonas aeruginosaType IV Pilus Secretin  Journal Articles
• Potent Antithrombin Activity and Delayed Clearance From the Circulation Characterize Recombinant Hirudin Genetically Fused to Albumin  Journal Articles
• Processing of the Papain Precursor  Journal Articles
• Proteolytic Cleavage of Ataxin-7 by Caspase-7 Modulates Cellular Toxicity and Transcriptional Dysregulation  Journal Articles
• Quorum sensing in Escherichia coli , Salmonella typhimurium , and Vibrio harveyi : A new family of genes responsible for autoinducer production  Journal Articles
• Rapid Insulin-like Growth Factor-1-induced Changes in Granulosa Cell Thrombospondin-1 Expression In Vitro  Journal Articles
• Reduction Precedes Cytidylyl Transfer without Substrate Channeling in Distinct Active Sites of the Bifunctional CDP-Ribitol Synthase from Haemophilus influenzae  Journal Articles
• Regulation of Smurf2 Ubiquitin Ligase Activity by Anchoring the E2 to the HECT Domain  Journal Articles
• Regulation of thrombospondin-1 expression through AU-rich elements in the 3′UTR of the mRNA  Journal Articles
• Repellent Signaling by Slit Requires the Leucine-Rich Repeats  Journal Articles
• Requirement of Lysine Residues Outside of the Proposed Pentasaccharide Binding Region for High Affinity Heparin Binding and Activation of Human Antithrombin III  Journal Articles
• RhoA Activation in Mesangial Cells by Mechanical Strain Depends on Caveolae and Caveolin-1 Interaction  Journal Articles
• Role of the Integrin-Binding Protein Osteopontin in Lymphatic Metastasis of Breast Cancer  Journal Articles
• Secretory expression and site‐directed mutagenesis studies of the winter flounder skin‐type antifreeze polypeptides  Journal Articles
• Selenite Inhibits the c-Jun N-terminal Kinase/Stress-activated Protein Kinase (JNK/SAPK) through a Thiol Redox Mechanism  Journal Articles
• Serine-401 as a batrachotoxin- and local anesthetic-sensing residue in the human cardiac Na+ channel  Journal Articles
• Signals for local and systemic responses of plants to pathogen attack  Journal Articles
• Site-directed mutagenesis and DNA sequence of pckA of Rhizobium NGR234, encoding phosphoenolpyruvate carboxykinase: gluconeogenesis and host-dependent symbiotic phenotype  Journal Articles
• Site‐directed mutagenesis of alanine‐382 of human antithrombin III  Journal Articles
• Site‐directed mutagenesis of the P2 residue of human antithrombin  Journal Articles
• Solution structure and dynamics of the outer membrane enzyme PagP by NMR  Journal Articles
• Spectinomycin kinase from Legionella pneumophila. Characterization of substrate specificity and identification of catalytically important residues  Journal Articles
• Structural Basis for Cyclic-Nucleotide Selectivity and cGMP-Selective Activation of PKG I  Journal Articles
• Structural Insights into the Regulation of Foreign Genes in Salmonella by the Hha/H-NS Complex  Journal Articles
• Structural basis for streptogramin B resistance in Staphylococcus aureus by virginiamycin B lyase  Journal Articles
• Structural basis for the emission of violet bioluminescence from a W92F obelin mutant  Journal Articles
• Structural modifications of outer membrane vesicles to refine them as vaccine delivery vehicles  Journal Articles
• Structure and Function of Sedoheptulose-7-phosphate Isomerase, a Critical Enzyme for Lipopolysaccharide Biosynthesis and a Target for Antibiotic Adjuvants  Journal Articles
• Structure-Activity Studies on Mammalian Adenosine Kinase  Journal Articles
• Structure-Function Analyses of Thrombomodulin by Gene-Targeting in Mice: The Cytoplasmic Domain Is Not Required for Normal Fetal Development  Journal Articles
• TATA-binding protein activates transcription when upstream of a GCN4-binding site in a novel yeast promoter.  Journal Articles
• Targeted mutagenesis of zebrafish antithrombin III triggers disseminated intravascular coagulation and thrombosis, revealing insight into function  Journal Articles
• The N-terminal domain of antithrombin-III is essential for heparin binding and complex-formation with, but not cleavage by, α-thrombin  Journal Articles
• The COOH terminus of aminoglycoside phosphotransferase (3')-IIIa is critical for antibiotic recognition and resistance  Journal Articles
• The Efficient Cellular Uptake of High Density Lipoprotein Lipids via Scavenger Receptor Class B Type I Requires Not Only Receptor-mediated Surface Binding but Also Receptor-specific Lipid Transfer Mediated by Its Extracellular Domain  Journal Articles
• The Functional Role of Conserved Acidic Residues of the Qcr7 Protein of the Cytochrome bc1 Complex in Saccharomyces cerevisiae  Journal Articles
• The Ice-Binding Site of Atlantic Herring Antifreeze Protein Corresponds to the Carbohydrate-Binding Site of C-Type Lectins  Journal Articles
• The PEA3 Subfamily of Ets Transcription Factors Synergizes with β-Catenin–LEF-1 To Activate Matrilysin Transcription in Intestinal Tumors  Journal Articles
• The Platform Protein Is Essential for Type IV Pilus Biogenesis  Journal Articles
• The Role of Ca²⁺-Coordinating Residues of Herring Antifreeze Protein in Antifreeze Activity  Journal Articles
• The Transactivation Function of the Pea3 Subfamily Ets Transcription Factors Is Regulated by Sumoylation  Journal Articles
• The Transcriptional Repressor ZEB Regulates p73 Expression at the Crossroad between Proliferation and Differentiation  Journal Articles
• The constitutive activation of the CEF-4/9E3 chemokine gene depends on C/EBPβ in v-src transformed chicken embryo fibroblasts  Journal Articles
• The efficient cellular uptake of high density lipoprotein lipids via scavenger receptor class B type I requires not only receptor-mediated surface binding but also receptor-specific lipid transfer mediated by its extracellular domain (vol 273, pg 26343, 1998)  Journal Articles
• The molecular basis of the expansive substrate specificity of the antibiotic resistance enzyme aminoglycoside acetyltransferase-6'-aminoglycoside phosphotransferase-2". The role of ASP-99 as an active site base important for acetyl transfer  Journal Articles
• The nerve growth factor precursor proNGF exhibits neurotrophic activity but is less active than mature nerve growth factor  Journal Articles
• The structural basis of molecular adaptation  Journal Articles
• Two Conserved Histidine Residues Are Critical to the Function of the TagF-like Family of Enzymes  Journal Articles
• Tyrosine 740 Phosphorylation of Discoidin Domain Receptor 2 by Src Stimulates Intramolecular Autophosphorylation and Shc Signaling Complex Formation  Journal Articles
• XPF with mutations in its conserved nuclease domain is defective in DNA repair but functions in TRF2-mediated telomere shortening  Journal Articles
• ndvF, a novel locus located on megaplasmid pRmeSU47b (pEXO) of Rhizobium meliloti, is required for normal nodule development  Journal Articles