Mutagenesis, Site-Directed Concept

subject area of

• A New Function of Human HtrA2 as an Amyloid-β Oligomerization Inhibitor  Academic Article
• A Thiolate Anion Buried within the Hydrocarbon Ruler Perturbs PagP Lipid Acyl Chain Selection  Academic Article
• A conserved MADS-box phosphorylation motif regulates differentiation and mitochondrial function in skeletal, cardiac, and smooth muscle cells  Academic Article
• A covalently linked recombinant albumin dimer is more rapidly cleared in vivo than are wild-type and mutant C34A albumin  Academic Article
• A critical role for the C-terminus of Nej1 protein in Lif1p association, DNA binding and non-homologous end-joining  Academic Article
• A glycosyl hydrolase activity of mammalian reovirus σ1 protein can contribute to viral infection through a mucus layer 1 1Edited by M. Yaniv  Academic Article
• A p56 Ligand Serves as a Coactivator of an Orphan Nuclear Hormone Receptor  Academic Article
• Active site labeling of the gentamicin resistance enzyme AAC(6')-APH(2") by the lipid kinase inhibitor wortmannin  Academic Article
• Aerial development in Streptomyces coelicolor requires sortase activity  Academic Article
• Altering Heparin Cofactor II at VAL439 (P6) either Impairs Inhibition of Thrombin or Confers Elastase Resistance  Academic Article
• Amino Acid Substitutions in the Herpes Simplex Virus Transactivator VP16 Uncouple Direct Protein-Protein Interaction and DNA Binding from Complex Assembly and Transactivation  Academic Article
• Amino acid substitutions of the P2 residue of human antithrombin that either enhance or impair function  Academic Article
• Amplification of TGFβ Induced ITGB6 Gene Transcription May Promote Pulmonary Fibrosis  Academic Article
• Application of Combinatorial Libraries and Protein Engineering to the Discovery of Novel Anti-Thrombotic Drugs  Conference Paper
• BCL11A represses HBG transcription in K562 cells  Academic Article
• BRAF status modulates Interelukin-8 expression through a CHOP-dependent mechanism in colorectal cancer  Academic Article
• Bax Homodimerization Is Not Required for Bax to Accelerate Chemotherapy-induced Cell Death  Academic Article
• Bcl-2 changes conformation to inhibit Bax oligomerization  Academic Article
• Binding Site Determinants for the LysR-Type Transcriptional Regulator PcaQ in the Legume Endosymbiont Sinorhizobium meliloti  Academic Article
• Catalytic Mechanism of Fungal Homoserine Transacetylase  Academic Article
• Characterization of a cryptic plasmid from Pseudomonas sp. and utilization of its temperature-sensitive derivatives for genetic manipulation  Academic Article
• Characterization of mice harboring a variant of EPCR with impaired ability to bind protein C: novel role of EPCR in hematopoiesis  Academic Article
• Characterization of yeast homoserine dehydrogenase, an antifungal target: the invariant histidine 309 is important for enzyme integrity  Academic Article
• Cleavage-dependent Ligation by the FLP Recombinase  Academic Article
• Common β-lactamases inhibit bacterial biofilm formation  Academic Article
• Computational Study of the Binding Modes of Caffeine to the Adenosine A_2A Receptor  Academic Article
• Conserved, unstructured regions in Pseudomonas aeruginosa PilO are important for type IVa pilus function  Academic Article
• Control of Centromere Localization of the MEI-S332 Cohesion Protection Protein  Academic Article
• Control of Motoneuron Survival by Angiogenin  Academic Article
• Crystal Structure of Anticoagulant Thrombin Variant E217K Provides Insights into Thrombin Allostery  Academic Article
• Crystal Structure of the Passenger Domain of the Escherichia coli Autotransporter EspP  Academic Article
• Cyclin B-dependent kinase 1 regulates human TRF1 to modulate the resolution of sister telomeres  Academic Article
• Cyclin-dependent Kinase 5 (Cdk5) Activation Domain of Neuronal Cdk5 Activator  Academic Article
• Cytokine-Induced Hepatic Apoptosis Is Dependent on FGL2/Fibroleukin: The Role of Sp1/Sp3 and STAT1/PU.1 Composite cis Elements  Academic Article
• Definition of a Consensus Transportin-specific Nucleocytoplasmic Transport Signal  Academic Article
• Determination of the origin-specific DNA-binding domain of polyomavirus large T antigen  Academic Article
• Different binding sites for the neuropeptide Y Y1 antagonists 1229U91 and J-104870 on human Y1 receptors  Academic Article
• Dissociation of Thrombin's Substrate Interactions Using Site-Directed Mutagenesis  Academic Article
• Distinct lipid effects on tBid and Bim activation of membrane permeabilization by pro-apoptotic Bax  Academic Article
• Distinct surfaces on Cdc5/PLK Polo-box domain orchestrate combinatorial substrate recognition during cell division  Academic Article
• Dynamic and tissue-specific proteolytic processing of chemerin in obese mice  Academic Article
• Effects of Double-Site Mutations of Vesicular Stomatitis Virus Glycoprotein G on Membrane Fusion Activity  Academic Article
• Effects of deletions in the carboxy-terminal hydrophobic region of herpes simplex virus glycoprotein gB on intracellular transport and membrane anchoring  Academic Article
• Effects of metal binding affinity on the chemical and thermal stability of site-directed mutants of rat oncomodulin  Academic Article
• Eliminating Competition: Characterizing and Eliminating Competitive Binding at Separate Sites between DAHP Synthase’s Essential Metal Ion and the Inhibitor DAHP Oxime  Academic Article
• Evidence That the Epidermal Growth Factor Receptor on Host Cells Confers Reovirus Infection Efficiency  Academic Article
• Evidence that Herpes Simplex Virus VP16 Is Required for Viral Egress Downstream of the Initial Envelopment Event  Academic Article
• Expression and purification of recombinant human receptor for advanced glycation endproducts in Escherichia coli  Academic Article
• Expression in a cell-free system of normal and variant forms of human antithrombin III. Ability to bind heparin and react with alpha-thrombin  Academic Article
• Factor VII Deficiency Caused by a Structural Variant N57D of the First Epidermal Growth Factor Domain  Academic Article
• Factors Determining Vesicular Lipid Mixing Induced by Shortened Constructs of Influenza Hemagglutinin  Academic Article
• Function and Redundancy of the Chaplin Cell Surface Proteins in Aerial Hypha Formation, Rodlet Assembly, and Viability in Streptomyces coelicolor  Academic Article
• Functional Analysis of Disulfide Linkages Clustered within the Amino Terminus of Human Apolipoprotein B  Academic Article
• Functional Mapping of the Surface Residues of Human Thrombin  Academic Article
• Functional role of conserved residues in the characteristic secretion NTPase motifs of the Pseudomonas aeruginosa type IV pilus motor proteins PilB, PilT and PilU  Academic Article
• Functionally important amino acids in Saccharomyces cerevisiae aspartate kinase  Academic Article
• Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3  Academic Article
• Heparin cofactor II is more sensitive than antithrombin to secretory impairment arising from mutations introduced into its carboxy-terminal region  Academic Article
• Homology Model of Dihydropyridine Receptor: Implications for L-type Ca2+ Channel Modulation by Agonists and Antagonists  Academic Article
• How Batrachotoxin Modifies the Sodium Channel Permeation Pathway: Computer Modeling and Site-Directed Mutagenesis  Academic Article
• HypF, a Carbamoyl Phosphate-converting Enzyme Involved in [NiFe] Hydrogenase Maturation  Academic Article
• IMPACT OF MUTATIONS AT THE P4 AND P5 POSITIONS ON THE REACTION OF ANTITHROMBIN WITH THROMBIN AND ELASTASE  Academic Article
• Identification and analysis of all components of a gel retardation assay by combination with immunoblotting.  Academic Article
• Identification of Critical Residues on Thrombin Mediating Its Interaction with Fibrin  Academic Article
• Identification of a cluster of residues in transmembrane segment 6 of domain III of the cockroach sodium channel essential for the action of pyrethroid insecticides  Academic Article
• Identification of the Catalytic Residues of AroA (Enolpyruvylshikimate 3-Phosphate Synthase) Using Partitioning Analysis  Academic Article
• Identification of the Factor VIIa Binding Site on Tissue Factor by Homologous Loop Swap and Alanine Scanning Mutagenesis  Academic Article
• Immunohistochemical staining for tilapia and human insulin demonstrates that a tilapia transgenic for humanized insulin is a mosaic  Academic Article
• Inscribing the Perimeter of the PagP Hydrocarbon Ruler by Site-Specific Chemical Alkylation  Academic Article
• Intracellular events determine the fate of antithrombin Utah  Academic Article
• Involvement of a calpain-like protease in the processing of the murine interleukin 1 alpha precursor  Academic Article
• Kinetic Analysis of a Unique Direct Prothrombinase, fgl2, and Identification of a Serine Residue Critical for the Prothrombinase Activity  Academic Article
• Lipid binding activity of a neuron-specific protein NAP-22 studied in vivo and in vitro  Academic Article
• Mechanism of allosteric inhibition in the Plasmodium falciparum cGMP-dependent protein kinase  Conference Paper
• Mechanism of aminoglycoside 3'-phosphotransferase type IIIa: His188 is not a phosphate-accepting residue  Academic Article
• Mechanism of aminoglycoside antibiotic kinase APH(3')-IIIa: role of the nucleotide positioning loop  Academic Article
• Modification of Pseudomonas aeruginosa Pa5196 Type IV Pilins at Multiple Sites with d -Ara f by a Novel GT-C Family Arabinosyltransferase, TfpW  Academic Article
• Modularity of protein function: Chimeric interleukin 1.beta.s containing specific protease inhibitor loops retain function of both molecules  Academic Article
• Modulation of Clearance of Recombinant Serum Albumin by Either Glycosylation or Truncation  Academic Article
• Molecular Mechanism for Lateral Lipid Diffusion between the Outer Membrane External Leaflet and a β-Barrel Hydrocarbon Ruler  Academic Article
• Molecular Mechanism of the Enterococcal Aminoglycoside 6‘-N-Acetyltransferase‘:  Role of GNAT-Conserved Residues in the Chemistry of Antibiotic Inactivation  Academic Article
• Molecular evidence for dual pyrethroid-receptor sites on a mosquito sodium channel  Academic Article
• Molecular mechanism of VanHst, an alpha-ketoacid dehydrogenase required for glycopeptide antibiotic resistance from a glycopeptide producing organism  Academic Article
• Molecular mechanism of aminoglycoside antibiotic kinase APH(3')-IIIa: roles of conserved active site residues  Academic Article
• Mutagenesis and peptide analysis of the DRY motif in the α2A adrenergic receptor: evidence for alternate mechanisms in G protein-coupled receptors  Academic Article
• Mutation of Any Site of N-Linked Glycosylation Accelerates the In Vivo Clearance of Recombinant Rabbit Antithrombin  Academic Article
• Mutational analysis of state-dependent contacts in the pore module of eukaryotic sodium channels  Academic Article
• Mutations in a Carboxy-Terminal Region of Vesicular Stomatitis Virus Glycoprotein G That Affect Membrane Fusion Activity  Academic Article
• Mutations of AKT3 are associated with a wide spectrum of developmental disorders including extreme megalencephaly  Academic Article
• NDPK-D (NM23-H4)-mediated externalization of cardiolipin enables elimination of depolarized mitochondria by mitophagy  Academic Article
• NMR Structures Reveal How Oxidation Inactivates Thrombomodulin  Academic Article
• NleG Type 3 Effectors from Enterohaemorrhagic Escherichia coli Are U-Box E3 Ubiquitin Ligases  Academic Article
• Nuclear import of the BTB/POZ transcriptional regulator Kaiso  Academic Article
• On the Interaction Between gp41 and Membranes: The Immunodominant Loop Stabilizes gp41 Helical Hairpin Conformation  Academic Article
• Pentavalent Ions Dependency Is a Conserved Property of Adenosine Kinase from Diverse Sources:  Identification of a Novel Motif Implicated in Phosphate and Magnesium Ion Binding and Substrate Inhibition  Academic Article
• Pervasive Cryptic Epistasis in Molecular Evolution  Academic Article
• PhoU Allows Rapid Adaptation to High Phosphate Concentrations by Modulating PstSCAB Transport Rate in Sinorhizobium meliloti  Academic Article
• Phosphorylation at the carboxy terminus of the 55-kilodalton adenovirus type 5 E1B protein regulates transforming activity  Academic Article
• PilF Is an Outer Membrane Lipoprotein Required for Multimerization and Localization of the Pseudomonas aeruginosa Type IV Pilus Secretin  Academic Article
• Polyphosphate binds with high affinity to exosite II of thrombin  Academic Article
• Potent Antithrombin Activity and Delayed Clearance From the Circulation Characterize Recombinant Hirudin Genetically Fused to Albumin  Academic Article
• Predominant Interactions between μ-Conotoxin Arg-13 and the Skeletal Muscle Na⁺ Channel Localized by Mutant Cycle Analysis  Academic Article
• Processing of the Papain Precursor  Academic Article
• Protein Engineering Thrombin for Optimal Specificity and Potency of Anticoagulant Activity in Vivo  Academic Article
• Proteolytic Cleavage of Ataxin-7 by Caspase-7 Modulates Cellular Toxicity and Transcriptional Dysregulation  Academic Article
• Quorum sensing in Escherichia coli , Salmonella typhimurium , and Vibrio harveyi : A new family of genes responsible for autoinducer production  Academic Article
• Rapid Insulin-like Growth Factor-1-induced Changes in Granulosa Cell Thrombospondin-1 Expression In Vitro  Academic Article
• Reduction Precedes Cytidylyl Transfer without Substrate Channeling in Distinct Active Sites of the Bifunctional CDP-Ribitol Synthase from Haemophilus influenzae  Academic Article
• Regulation of Smurf2 Ubiquitin Ligase Activity by Anchoring the E2 to the HECT Domain  Academic Article
• Regulation of thrombospondin-1 expression through AU-rich elements in the 3′UTR of the mRNA  Academic Article
• Repellent Signaling by Slit Requires the Leucine-Rich Repeats  Academic Article
• Requirement of Lysine Residues Outside of the Proposed Pentasaccharide Binding Region for High Affinity Heparin Binding and Activation of Human Antithrombin III  Academic Article
• RhoA Activation in Mesangial Cells by Mechanical Strain Depends on Caveolae and Caveolin-1 Interaction  Academic Article
• Role of the Integrin-Binding Protein Osteopontin in Lymphatic Metastasis of Breast Cancer  Academic Article
• Serine-401 as a batrachotoxin- and local anesthetic-sensing residue in the human cardiac Na+ channel  Academic Article
• Signals for local and systemic responses of plants to pathogen attack  Academic Article
• Site-directed mutagenesis and DNA sequence of pckA of Rhizobium NGR234, encoding phosphoenolpyruvate carboxykinase: gluconeogenesis and host-dependent symbiotic phenotype  Academic Article
• Site-directed mutagenesis of alanine-382 of human antithrombin III  Academic Article
• Site-directed mutagenesis of the P2 residue of human antithrombin  Academic Article
• Solution structure and dynamics of the outer membrane enzyme PagP by NMR  Academic Article
• Spectinomycin kinase from Legionella pneumophila. Characterization of substrate specificity and identification of catalytically important residues  Academic Article
• Structural Basis for Cyclic-Nucleotide Selectivity and cGMP-Selective Activation of PKG I  Academic Article
• Structural Insights into the Regulation of Foreign Genes in Salmonella by the Hha/H-NS Complex  Academic Article
• Structural basis for streptogramin B resistance in Staphylococcus aureus by virginiamycin B lyase  Academic Article
• Structural basis for the emission of violet bioluminescence from a W92F obelin mutant  Academic Article
• Structural modifications of outer membrane vesicles to refine them as vaccine delivery vehicles  Academic Article
• Structural requirements for the activation of human factor VIII by thrombin  Academic Article
• Structure and Activity of the Metal-independent Fructose-1,6-bisphosphatase YK23 from Saccharomyces cerevisiae  Academic Article
• Structure and Function of Sedoheptulose-7-phosphate Isomerase, a Critical Enzyme for Lipopolysaccharide Biosynthesis and a Target for Antibiotic Adjuvants  Academic Article
• Structure-Activity Studies on Mammalian Adenosine Kinase  Academic Article
• Structure-Function Analyses of Thrombomodulin by Gene-Targeting in Mice: The Cytoplasmic Domain Is Not Required for Normal Fetal Development  Academic Article
• TATA-binding protein activates transcription when upstream of a GCN4-binding site in a novel yeast promoter.  Academic Article
• Targeted mutagenesis of zebrafish antithrombin III triggers disseminated intravascular coagulation and thrombosis, revealing insight into function  Academic Article
• The N-terminal domain of antithrombin-III is essential for heparin binding and complex-formation with, but not cleavage by, α-thrombin  Academic Article
• The COOH terminus of aminoglycoside phosphotransferase (3')-IIIa is critical for antibiotic recognition and resistance  Academic Article
• The Efficient Cellular Uptake of High Density Lipoprotein Lipids via Scavenger Receptor Class B Type I Requires Not Only Receptor-mediated Surface Binding but Also Receptor-specific Lipid Transfer Mediated by Its Extracellular Domain  Academic Article
• The Functional Role of Conserved Acidic Residues of the Qcr7 Protein of the Cytochrome bc1 Complex in Saccharomyces cerevisiae  Academic Article
• The Guanylyltransferase Domain of Mammalian mRNA Capping Enzyme Binds to the Phosphorylated Carboxyl-terminal Domain of RNA Polymerase II  Academic Article
• The Membrane-Proximal Region of Vesicular Stomatitis Virus Glycoprotein G Ectodomain Is Critical for Fusion and Virus Infectivity  Academic Article
• The PEA3 Subfamily of Ets Transcription Factors Synergizes with β-Catenin–LEF-1 To Activate Matrilysin Transcription in Intestinal Tumors  Academic Article
• The Platform Protein Is Essential for Type IV Pilus Biogenesis  Academic Article
• The Transactivation Function of the Pea3 Subfamily Ets Transcription Factors Is Regulated by Sumoylation  Academic Article
• The Transcriptional Repressor ZEB Regulates p73 Expression at the Crossroad between Proliferation and Differentiation  Academic Article
• The constitutive activation of the CEF-4/9E3 chemokine gene depends on C/EBPβ in v-src transformed chicken embryo fibroblasts  Academic Article
• The molecular basis of the expansive substrate specificity of the antibiotic resistance enzyme aminoglycoside acetyltransferase-6'-aminoglycoside phosphotransferase-2". The role of ASP-99 as an active site base important for acetyl transfer  Academic Article
• The nerve growth factor precursor proNGF exhibits neurotrophic activity but is less active than mature nerve growth factor  Academic Article
• The structural basis of molecular adaptation  Academic Article
• Thrombin Activation of Factor XI on Activated Platelets Requires the Interaction of Factor XI and Platelet Glycoprotein Ibα with Thrombin Anion-binding Exosites I and II, Respectively  Academic Article
• Two Conserved Histidine Residues Are Critical to the Function of the TagF-like Family of Enzymes  Academic Article
• XPF with mutations in its conserved nuclease domain is defective in DNA repair but functions in TRF2-mediated telomere shortening  Academic Article
• ndvF, a novel locus located on megaplasmid pRmeSU47b (pEXO) of Rhizobium meliloti, is required for normal nodule development  Academic Article