Journal article
Functional role of conserved residues in the characteristic secretion NTPase motifs of the Pseudomonas aeruginosa type IV pilus motor proteins PilB, PilT and PilU
Abstract
Type IV pili are retractable protein fibres used by many bacterial pathogens for adherence, twitching motility, biofilm development and host colonization. In Pseudomonas aeruginosa, PilB and PilT are bipolar proteins belonging to the secretion NTPase superfamily, and power pilus extension and retraction, respectively, while the unipolar PilT paralogue PilU supports pilus retraction in an unknown manner. Assay of purified 6xHis-tagged PilB, PilT …
Authors
Chiang P; Sampaleanu LM; Ayers M; Pahuta M; Howell PL; Burrows LL
Journal
Microbiology, Vol. 154, No. 1, pp. 114–126
Publisher
Microbiology Society
Publication Date
January 1, 2008
DOI
10.1099/mic.0.2007/011320-0
ISSN
1350-0872
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Adenosine TriphosphatasesAmino Acid MotifsAmino Acid SequenceBacterial ProteinsConserved SequenceFimbriae ProteinsGenetic Complementation TestLocomotionModels, MolecularMolecular Motor ProteinsMolecular Sequence DataMutagenesis, Site-DirectedNucleoside-TriphosphataseOxidoreductasesPseudomonas aeruginosa