Journal article
Characterization of a cryptic plasmid from Pseudomonas sp. and utilization of its temperature-sensitive derivatives for genetic manipulation
Abstract
A new cryptic plasmid, named pMM101, in an environmental Pseudomonas sp. has been isolated, and its 2140-bp DNA sequence has been determined and analyzed. One open reading frame (Rep(MM)) similar to the rep gene of the rolling-circle replicon (RCR) group VIII has been allocated in the replication region where a putative double-strand origin dso and single-strand origin sso could also be identified. After in vitro mutagenesis by error-prone PCR …
Authors
Chen L; Wang W; Sun W; Surette M; Duan K
Journal
Plasmid, Vol. 64, No. 2, pp. 110–117
Publisher
Elsevier
Publication Date
September 2010
DOI
10.1016/j.plasmid.2010.05.003
ISSN
0147-619X