Journal article
Site-directed mutagenesis and DNA sequence of pckA of Rhizobium NGR234, encoding phosphoenolpyruvate carboxykinase: gluconeogenesis and host-dependent symbiotic phenotype
Abstract
We have cloned and sequenced the pckA gene of Rhizobium sp. NGR234, a broad host-range strain. The gene encodes phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme of gluconeogenesis. The locus was isolated and subcloned from a genomic library of NGR234 employing hybridization with an R. meliloti pck gene probe and complementation of a Tn5 mutant in this species. The DNA sequence of pckA (NGR234) was determined and encoded a PEPCK protein …
Authors
Østerås M; Finan TM; Stanley J
Journal
Molecular Genetics and Genomics, Vol. 230, No. 1-2, pp. 257–269
Publisher
Springer Nature
Publication Date
November 1991
DOI
10.1007/bf00290676
ISSN
1617-4615
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Amino Acid SequenceBase SequenceCloning, MolecularDNA, BacterialFabaceaeGene Expression Regulation, BacterialGene Expression Regulation, EnzymologicGenes, BacterialGluconeogenesisMolecular Sequence DataMutagenesis, Site-DirectedNucleic Acid ConformationNucleic Acid HybridizationPhenotypePhosphoenolpyruvate Carboxykinase (GTP)Plants, MedicinalRNA, BacterialRNA, MessengerRestriction MappingRhizobiumSequence AlignmentSymbiosisTranscription, Genetic