Journal article
Characterization of yeast homoserine dehydrogenase, an antifungal target: the invariant histidine 309 is important for enzyme integrity
Abstract
Fungal homoserine dehydrogenase (HSD) is required for the biosynthesis of threonine, isoleucine and methionine from aspartic acid, and is a target for antifungal agents. HSD from the yeast Saccharomyces cerevisiae was overproduced in Escherichia coli and 25 mg of soluble dimeric enzyme was purified per liter of cell culture in two steps. HSD efficiently reduces aspartate semialdehyde to homoserine (Hse) using either NADH or NADPH with kcat/Km …
Authors
Jacques SL; Nieman C; Bareich D; Broadhead G; Kinach R; Honek JF; Wright GD
Journal
Biochimica Et Biophysica Acta, Vol. 1544, No. 1-2, pp. 28–41
Publication Date
January 12, 2001
ISSN
0006-3002