Journal article
ERK1 and ERK2 kinases activate hydroxyurea-induced S-phase checkpoint in MCF7 cells by mediating ATR activation
Abstract
Modulation of MEK has been demonstrated to affect hydroxyurea (HU) induced-DNA damage response (DDR), implying the involvement of ERK1 and ERK2 in the process. To directly examine how the ERK kinases function in HU-initiated DDR, we knocked-down either ERK1 or ERK2 in MCF7 cells. This resulted in reduction of HU-induced phosphorylation of CHK1 S345 (serine 345), p53 S15, and H2AX S139. While HU potently induced CDC2 Y15 (tyrosine 15) …
Authors
Wei F; Xie Y; He L; Tao L; Tang D
Journal
Cellular Signalling, Vol. 23, No. 1, pp. 259–268
Publisher
Elsevier
Publication Date
January 2011
DOI
10.1016/j.cellsig.2010.09.010
ISSN
0898-6568
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Ataxia Telangiectasia Mutated ProteinsButadienesCDC2 Protein KinaseCell Cycle ProteinsCell Line, TumorCell NucleusCheckpoint Kinase 1DNA DamageHistonesHumansHydroxyureaMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3MitosisNitrilesPhosphorylationProtein KinasesProtein Serine-Threonine KinasesRNA InterferenceS Phase