Journal article
The solution structure of Lac repressor headpiece 62 complexed to a symmetrical lac operator
Abstract
BACKGROUND: Lactose repressor protein (Lac) controls the expression of the lactose metabolic genes in Escherichia coli by binding to an operator sequence in the promoter of the lac operon. Binding of inducer molecules to the Lac core domain induces changes in tertiary structure that are propagated to the DNA-binding domain through the connecting hinge region, thereby reducing the affinity for the operator. Protein-protein and protein-DNA …
Authors
Spronk CA; Bonvin AM; Radha PK; Melacini G; Boelens R; Kaptein R
Journal
Structure, Vol. 7, No. 12, pp. 1483–14s3
Publisher
Elsevier
Publication Date
January 1999
DOI
10.1016/s0969-2126(00)88339-2
ISSN
0969-2126
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Bacterial ProteinsBase SequenceBinding SitesCrystallography, X-RayDNA, BacterialDimerizationEscherichia coliEscherichia coli ProteinsHydrogen BondingLac OperonLac RepressorsMolecular Sequence DataNuclear Magnetic Resonance, BiomolecularNucleic Acid ConformationOligodeoxyribonucleotidesPromoter Regions, GeneticProtein ConformationProtein Structure, SecondaryRepressor Proteins