Journal article
Massively parallel enzyme kinetics reveals the substrate recognition landscape of the metalloprotease ADAMTS13
Abstract
Proteases play important roles in many biologic processes and are key mediators of cancer, inflammation, and thrombosis. However, comprehensive and quantitative techniques to define the substrate specificity profile of proteases are lacking. The metalloprotease ADAMTS13 regulates blood coagulation by cleaving von Willebrand factor (VWF), reducing its procoagulant activity. A mutagenized substrate phage display library based on a 73-amino acid …
Authors
Kretz CA; Dai M; Soylemez O; Yee A; Desch KC; Siemieniak D; Tomberg K; Kondrashov FA; Meng F; Ginsburg D
Journal
Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, No. 30, pp. 9328–9333
Publisher
Proceedings of the National Academy of Sciences
Publication Date
July 28, 2015
DOI
10.1073/pnas.1511328112
ISSN
0027-8424