Isolation, Sequence, Infectivity, and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2 Academic Article uri icon

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abstract

  • Since its emergence in Wuhan, China, in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected ≈6 million persons worldwide. As SARS-CoV-2 spreads across the planet, we explored the range of human cells that can be infected by this virus. We isolated SARS-CoV-2 from 2 infected patients in Toronto, Canada; determined the genomic sequences; and identified single-nucleotide changes in representative populations of our virus stocks. We also tested a wide range of human immune cells for productive infection with SARS-CoV-2. We confirm that human primary peripheral blood mononuclear cells are not permissive for SARS-CoV-2. As SARS-CoV-2 continues to spread globally, it is essential to monitor single-nucleotide polymorphisms in the virus and to continue to isolate circulating viruses to determine viral genotype and phenotype by using in vitro and in vivo infection models.

authors

  • Banerjee, Arinjay
  • Nasir, Jalees A
  • Budylowski, Patrick
  • Yip, Lily
  • Aftanas, Patryk
  • Christie, Natasha
  • Ghalami, Ayoob
  • Baid, Kaushal
  • Raphenya, Amogelang R
  • Hirota, Jeremy A
  • Miller, Matthew S
  • McGeer, Allison J
  • Ostrowski, Mario
  • Kozak, Robert A
  • McArthur, Andrew G
  • Mossman, Karen
  • Mubareka, Samira

publication date

  • September 2020