Journal article
Three rhamnosyltransferases responsible for assembly of the A‐band D‐rhamnan polysaccharide in Pseudomonas aeruginosa: a fourth transferase, WbpL, is required for the initiation of both A‐band and B‐band lipopolysaccharide synthesis
Abstract
The Pseudomonas aeruginosa A-band lipopolysaccharide (LPS) molecule has an O-polysaccharide region composed of trisaccharide repeat units of alpha1-->2, alpha1-->3, alpha1-->3 linked D-rhamnose (Rha). The A-band polysaccharide is assembled by the alpha-D-rhamnosyltransferases, WbpX, WbpY and WbpZ. WbpZ probably transfers the first Rha residue onto the A-band accepting molecule, while WbpY and WbpX subsequently transfer two alpha1-->3 linked Rha …
Authors
Rocchetta HL; Burrows LL; Pacan JC; Lam JS
Journal
Molecular Microbiology, Vol. 28, No. 6, pp. 1103–1119
Publisher
Wiley
Publication Date
June 1998
DOI
10.1046/j.1365-2958.1998.00871.x
ISSN
0950-382X
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Adenosine TriphosphatasesAmino Acid SequenceBacterial ProteinsBase SequenceBlotting, SouthernBlotting, WesternCarbohydrate SequenceCloning, MolecularDNA HelicasesElectrophoresis, Polyacrylamide GelEscherichia coliEscherichia coli ProteinsGenes, BacterialGenetic Complementation TestGlycosyltransferasesLipopolysaccharidesMolecular Sequence DataMultigene FamilyMutationO AntigensPlasmidsPseudomonas aeruginosaRestriction MappingSequence AlignmentSequence Homology, Amino AcidSerotyping