Journal article
Enhanced HER-2/neu-specific antitumor immunity by cotransduction of mouse dendritic cells with two genes encoding HER-2/neu and alpha tumor necrosis factor
Abstract
The present study uses an in vivo murine tumor model expressing the human HER-2/neu antigen to evaluate the potential vaccine using dendritic cells (DCs) infected with adenovirus AdVHER-2. We first investigated whether infected DCs (DCHER-2) engineered to express HER-2/neu could induce HER-2/neu-specific immune responses. Our data showed that (i) AdVHER2-infected DCHER-2 expressed HER-2/neu by Western blot and flow cytometric analysis, and (ii) …
Authors
Chen Z; Huang H; Chang T; Carlsen S; Saxena A; Marr R; Xing Z; Xiang J
Journal
Cancer Gene Therapy, Vol. 9, No. 9, pp. 778–786
Publisher
Springer Nature
Publication Date
September 1, 2002
DOI
10.1038/sj.cgt.7700498
ISSN
0929-1903
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AdenoviridaeAnimalsBlotting, WesternCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCancer VaccinesCytotoxicity Tests, ImmunologicDNA PrimersDendritic CellsFemaleFlow CytometryGenetic VectorsIntercellular Adhesion Molecule-1Lac OperonMiceMice, Inbred BALB CNeoplasms, ExperimentalReceptor, ErbB-2Reverse Transcriptase Polymerase Chain ReactionTransduction, GeneticTransfectionTumor Necrosis Factor-alpha