Journal article
An Initial Reaction Rate Assay for "Glycerate Dehydrogenase"
Abstract
Abstract
Concentrations of substrate, coenzyme, and hydrogen ion, optimal for the reduction of hydroxypyruvate at 37°C by normal and pathological human sera are described. We confirmed, by using the supernatant fluid from homogenized human heart and liver tissue, that the faster-moving isoenzymes have a greater affinity for substrate and coenzyme than the slowermoving isoenzymes, which require higher concentrations for …
Authors
McQueen MJ; King J
Journal
Clinical Chemistry, Vol. 17, No. 11, pp. 1089–1092
Publisher
Oxford University Press (OUP)
Publication Date
November 1, 1971
DOI
10.1093/clinchem/17.11.1089
ISSN
0009-9147