Journal article
A barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networks
Abstract
Heterodimeric capping protein (CP/CapZ) is an essential factor for the assembly of branched actin networks, which push against cellular membranes to drive a large variety of cellular processes. Aside from terminating filament growth, CP potentiates the nucleation of actin filaments by the Arp2/3 complex in branched actin networks through an unclear mechanism. Here, we combine structural biology with in vitro reconstitution to demonstrate that …
Authors
Funk J; Merino F; Schaks M; Rottner K; Raunser S; Bieling P
Journal
Nature Communications, Vol. 12, No. 1,
Publisher
Springer Nature
DOI
10.1038/s41467-021-25682-5
ISSN
2041-1723
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Actin Capping ProteinsActin CytoskeletonActin-Related Protein 2-3 ComplexActinsAnimalsBinding SitesCattleCytoskeletonGelsolinGene Expression RegulationHumansIntercellular Signaling Peptides and ProteinsKineticsMelanocytesMelanoma, ExperimentalMiceModels, MolecularProfilinsProtein BindingProtein Conformation, alpha-HelicalProtein Conformation, beta-StrandProtein Interaction Domains and MotifsRecombinant ProteinsThymus GlandWiskott-Aldrich Syndrome Protein, Neuronal