Effects of Activators of Different Intracellular Signaling Pathways on Steroid Production by Goldfish Vitellogenic Ovarian Follicles

For the present study, calcium ionophore A23187 and phorbol 12-myristate 13-acetate (PMA) were used to investigate the possible roles that changes in intracellular calcium content and protein kinase C activation play in steroid production by goldfish vitellogenic ovarian follicles (0.2-0.7 mm in diameter) incubated in vitro. While largely ineffective when tested alone, PMA and A23187 exhibit synergism leading to increased testosterone and 17 …