Journal article
Characterization of CHO-K1 cells stably expressing PDE-IV enzymes
Abstract
A CHO-K1 cell line stably expressing a recombinant full-length human PDE-IVa (HSPDE4A4B) enzyme was established under hygromycin B selection. Full-length expression of the protein was determined by Western blot analysis, which revealed the presence of a 125-kDa immunoreactive band using rabbit anti-PDE-IVa antibodies. The potency of inhibitor compounds was examined by their ability to increase cAMP in the whole-cell, and by their ability to …
Authors
Pon DJ; Plant M; Tkach J; Boulet L; Muise E; Allen RA; Rodger IW
Journal
Cell Biochemistry and Biophysics, Vol. 29, No. 1-2, pp. 159–178
Publisher
Springer Nature
Publication Date
February 1998
DOI
10.1007/bf02737834
ISSN
1085-9195