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Functional differences in the cytochrome P450 1...
Journal article

Functional differences in the cytochrome P450 1 family enzymes from Zebrafish (Danio rerio) using heterologously expressed proteins

Abstract

Mammalian cytochrome P450 1 (CYP1) genes are well characterized, but in other vertebrates only the functions of CYP1A genes have been well studied. We determined the catalytic activity of zebrafish CYP1A, CYP1B1, CYP1C1, CYP1C2, and CYP1D1 proteins using 11 fluorometric substrates and benzo[a]pyrene (BaP). The resorufin-based substrates, 7-ethoxyresorufin, 7-methoxyresorufin, and 7-benzyloxyresorufin, were well metabolized by all CYP1s except …

Authors

Scornaienchi ML; Thornton C; Willett KL; Wilson JY

Journal

Archives of Biochemistry and Biophysics, Vol. 502, No. 1, pp. 17–22

Publisher

Elsevier

Publication Date

October 2010

DOI

10.1016/j.abb.2010.06.018

ISSN

0003-9861