Journal article
UDP-N-acetylmuramic Acid (UDP-MurNAc) Is a Potent Inhibitor of MurA (Enolpyruvyl-UDP-GlcNAc Synthase) †
Abstract
Purified recombinant MurA (enolpyruvyl-UDP-GlcNAc synthase) overexpressed in Escherichia coli had significant amounts of UDP-MurNAc (UDP-N-acetylmuramic acid) bound after purification. UDP-MurNAc is the product of MurB, the next enzyme in peptidoglycan biosynthesis. About 25% of MurA was complexed with UDP-MurNAc after five steps during purification that should have removed it. UDP-MurNAc isolated from MurA was identified by mass spectrometry, …
Authors
Mizyed S; Oddone A; Byczynski B; Hughes DW; Berti PJ
Journal
Biochemistry, Vol. 44, No. 10, pp. 4011–4017
Publisher
American Chemical Society (ACS)
Publication Date
March 1, 2005
DOI
10.1021/bi047704w
ISSN
0006-2960