Journal article
Functional Screening of Cytochrome P450 Activity and Uncoupling by Capillary Electrophoresis
Abstract
Cytochrome P450s (CYPs) are functionally diverse monooxygenases responsible for oxidation of endogenous and xenobiotic compounds. The function of nonmammalian CYPs are largely unknown and tools for characterization limited. CYPs critical for xenobiotic metabolism are prone to catalytic cycle uncoupling resulting in reactive oxygen species (ROS) generation that is highly dependent on the specific CYP isoform and substrate interaction. This study …
Authors
Harskamp J; Britz-McKibbin P; Wilson JY
Journal
Analytical Chemistry, Vol. 84, No. 2, pp. 862–866
Publisher
American Chemical Society (ACS)
Publication Date
January 17, 2012
DOI
10.1021/ac202787n
ISSN
0003-2700