Purification and Characterization of Two Haloperoxidases from the Glycopeptide Antibiotic ProducerStreptomyces toyocaensisNRRL 15009

Streptomyces toyocaensis NRRL 15009 produces A47934, a glycopeptide antibiotic. This compound is composed of several unusual amino acids, some of which have chlorinated aromatic rings. We have isolated two distinct halogenating enzymes, a chloroperoxidase (42,882 Da) and a catalase/bromoperoxidase (53,890 Da), from late log phase drug producing cultures of this organism grown on soy based media. Both these enzymes are azide sensitive and show …