Journal article
Use of differential fluorescence induction and optical trapping to isolate environmentally induced genes
Abstract
The techniques of differential fluorescence induction (DFI) and optical trapping (OT) have been combined to allow the identification of environmentally induced genes in single bacterial cells. Designated DFI-OT, this technique allows the in situ isolation of genes driving the expression of green fluorescent protein (Gfp) using temporal and spatial criteria. A series of plasmid-based promoter probe vectors (pOT) was developed for the …
Authors
Allaway D; Schofield NA; Leonard ME; Gilardoni L; Finan TM; Poole PS
Journal
Environmental Microbiology, Vol. 3, No. 6, pp. 397–406
Publisher
Wiley
Publication Date
June 2001
DOI
10.1046/j.1462-2920.2001.00205.x
ISSN
1462-2912