Journal article
Sedimentation Analysis of DNA from Irradiated and Unirradiated L Cells
Abstract
DNA, released from unirradiated mouse L-cells gently lysed in a thin layer of 2% sucrose on top of an alkaline sucrose gradient, was found to sediment in a narrow band with a sedimentation coefficient of about 500S. Exposure of cells to increasing doses of X-rays (89-712 rads) continuously reduced the DNA sedimentation velocity until, after about 890 rads, the DNA appeared in a narrow peak with a sedimentation coefficient of approximately 180S. …
Authors
McBurney MW; Graham FL; Whitmore GF
Journal
Biophysical Journal, Vol. 12, No. 4, pp. 369–383
Publisher
Elsevier
Publication Date
4 1972
DOI
10.1016/s0006-3495(72)86090-9
ISSN
0006-3495