Ancient Whole Genome Enrichment Using Baits Built from Modern DNA Journal Articles uri icon

  •  
  • Overview
  •  
  • Research
  •  
  • Identity
  •  
  • Additional Document Info
  •  
  • View All
  •  

abstract

  • We report metrics from complete genome capture of nuclear DNA from extinct mammoths using biotinylated RNAs transcribed from an Asian elephant DNA extract. Enrichment of the nuclear genome ranged from 1.06- to 18.65-fold, to an apparent maximum threshold of ∼80% on-target. This projects an order of magnitude less costly complete genome sequencing from long-dead organisms, even when a reference genome is unavailable for bait design.

authors

  • Enk, Jacob M
  • Devault, Alison M
  • Kuch, Melanie
  • Murgha, Yusuf E
  • Rouillard, Jean-Marie
  • Poinar, Hendrik

publication date

  • May 2014