Journal article
The IgE-facilitated allergen binding (FAB) assay: Validation of a novel flow-cytometric based method for the detection of inhibitory antibody responses
Abstract
The IgE-facilitated allergen binding (IgE-FAB) assay represents an in vitro model of facilitated allergen presentation. Allergen-IgE complexes are incubated with an EBV-transformed B cell line and complexes bound to CD23 on the surface of cells are detected by flow cytometry. The addition of serum from patients who have received allergen-specific immunotherapy has been shown previously to inhibit allergen-IgE complex binding to CD23 on B cells. …
Authors
Shamji MH; Wilcock LK; Wachholz PA; Dearman RJ; Kimber I; Wurtzen PA; Larché M; Durham SR; Francis JN
Journal
Journal of Immunological Methods, Vol. 317, No. 1-2, pp. 71–79
Publisher
Elsevier
Publication Date
December 2006
DOI
10.1016/j.jim.2006.09.004
ISSN
0022-1759
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AllergensAntibody FormationAntigen PresentationB-LymphocytesDesensitization, ImmunologicDouble-Blind MethodFlow CytometryHumansImmunoglobulin EPlacebosPoaceaePollenProtein BindingROC CurveRandomized Controlled Trials as TopicReceptors, IgEReproducibility of ResultsRhinitis, Allergic, SeasonalSensitivity and Specificity