Journal article
Structure and Function of APH(4)-Ia, a Hygromycin B Resistance Enzyme* ♦
Abstract
The aminoglycoside phosphotransferase (APH) APH(4)-Ia is one of two enzymes responsible for bacterial resistance to the atypical aminoglycoside antibiotic hygromycin B (hygB). The crystal structure of APH(4)-Ia enzyme was solved in complex with hygB at 1.95 Å resolution. The APH(4)-Ia structure adapts a general two-lobe architecture shared by other APH enzymes and eukaryotic kinases, with the active site located at the interdomain cavity. The …
Authors
Stogios PJ; Shakya T; Evdokimova E; Savchenko A; Wright GD
Journal
Journal of Biological Chemistry, Vol. 286, No. 3, pp. 1966–1975
Publisher
Elsevier
Publication Date
January 2011
DOI
10.1074/jbc.m110.194266
ISSN
0021-9258