Oligonucleotide degradation contributes to resistance to antisense compounds Journal Articles uri icon

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  • A subline of the human B cell lymphoma DHL-4, grown in the artificial serum-free medium HB101, displayed a resistant phenotype to the activity of an antisense oligodeoxynucleotide (aODN) effective on the parental DHL-4 line. It was found that the cellular uptake of the 18mer aODN in the two cell lines was almost the same. In contrast, the unresponsive subline DHL-4r degraded the aODN very efficiently, in contrast to the stability of aODN inside cells of the parental DHL-4 line. Activation of the degrading 'machinery' combined with selective properties of the artificial medium may be responsible for the loss of responsiveness to aODN.


  • Alama, Stanley
  • Ryte, A
  • Morelli, S
  • Mazzei, M
  • Alama, A
  • Franco, P
  • Canti, GF
  • Nicolin, A

publication date

  • April 1993