Journal article
Antibodies to rat soluble IL‐6 receptor stimulate B9 hybridoma cell proliferation
Abstract
Interleukin-6 mediates its pleiotropic effects by interacting with its membrane bound receptor (gp80) or the soluble counterpart gp54, resulting in activation of a complex that includes the transducer protein gp130. We have generated a polyclonal antibody against the rat soluble IL-6 receptor (anti-rat sIL-6R) in rabbits. By Western blot analysis we show that purified anti-rat sIL-6R IgG antibody reacts specifically with recombinant rat sIL-6R …
Authors
Thibault V; Richards CD; Botelho F; Gauldie J
Journal
FEBS Letters, Vol. 408, No. 2, pp. 182–186
Publisher
Wiley
Publication Date
May 19, 1997
DOI
10.1016/s0014-5793(97)00417-1
ISSN
0014-5793
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Acute-Phase ProteinsAnimalsAntibodiesAntigens, CDCell DivisionCell LineCysteine Proteinase InhibitorsDNA-Binding ProteinsHumansHybridomasImmunoglobulin Fab FragmentsImmunoglobulin GInterleukin-6Oncostatin MPeptidesRabbitsRatsReceptors, InterleukinReceptors, Interleukin-6Recombinant ProteinsSTAT1 Transcription FactorSTAT3 Transcription FactorSignal TransductionSolubilityTrans-ActivatorsTumor Cells, Cultured