Polyomavirus large T antigen binds independently to multiple, unique regions on the viral genome

To map the polyomavirus large T antigen binding sites on the viral genome we employed a quantitative immunoassay. Defined, radiolabeled fragments of the viral genome were reacted with crude nuclear extracts prepared from lytically infected mouse 3T6 cells, and the fragments bound by large T antigen were immunoprecipitated with anti-T serum and Formalin-fixed Staphylococcus aureus. The immunoprecipitated DNA was then analyzed by gel …