Journal article
Identification and immunocytochemical localization of two different carbonic anhydrase isoenzymes in teleostean fish erythrocytes and gill epithelia
Abstract
Carbonic anhydrase was purified from the gills (CAB) of the rainbow trout Salmo gairdneri and from erythrocytes (CAE) of the fresh water carp Cyprinus carpio. The purification of the isozymes was confirmed by SDS acrylamide gel electrophoresis. Antibodies against the purified CAB and CAE were then raised in rabbits. Specificity was verified by immunoblotting. No cross-reaction was found between them, using the immunodot technique. CAB antiserum …
Authors
Rahim SM; Delaunoy J-P; Laurent P
Journal
Histochemistry and Cell Biology, Vol. 89, No. 5, pp. 451–459
Publisher
Springer Nature
Publication Date
September 1988
DOI
10.1007/bf00492602
ISSN
0948-6143