Journal article
Purification and characterization of VanR and the cytosolic domain of VanS: a two-component regulatory system required for vancomycin resistance in Enterococcus faecium BM4147.
Abstract
Resistance to the glycopeptide antibiotic vancomycin requires five genes. Two of these, vanR and vanS, have sequence homology to cytoplasmic response regulatory (VanR) and transmembrane sensory (VanS) proteins of two-component regulatory systems used to sense and transduce environmental signals. We report the overproduction and purification to homogeneity of VanR (27 kDa) and of a fusion protein of VanS (residues 95-374, the cytosolic domain) …
Authors
Wright GD; Holman TR; Walsh CT
Journal
Biochemistry, Vol. 32, No. 19, pp. 5057–5063
Publisher
American Chemical Society (ACS)
Publication Date
May 18, 1993
DOI
10.1021/bi00070a013
ISSN
0006-2960
Fields of Research (FoR)
Medical Subject Headings (MeSH)
ATP-Binding Cassette TransportersAdenosine DiphosphateAdenosine TriphosphateBacterial ProteinsCarrier ProteinsDrug Resistance, MicrobialEnterococcus faeciumEscherichia coliEscherichia coli ProteinsGene ExpressionHistidineHydrolysisKineticsMaltose-Binding ProteinsMonosaccharide Transport ProteinsPhosphorylationPolymerase Chain ReactionProtein KinasesRecombinant Fusion ProteinsSignal TransductionTranscription FactorsTransformation, BacterialVancomycin