Journal article
Allosteric Site Variants of Haemophilus influenzae β-Carbonic Anhydrase
Abstract
Haemophilus influenzae beta-carbonic anhydrase (HICA) is hypothesized to be an allosteric protein that is regulated by the binding of bicarbonate ion to a non-catalytic (inhibitory) site that controls the ligation of Asp44 to the catalytically essential zinc ion. We report here the X-ray crystallographic structures of two variants (W39F and Y181F) involved in the binding of bicarbonate ion in the non-catalytic site and an active-site variant …
Authors
Rowlett RS; Tu C; Lee J; Herman AG; Chapnick DA; Shah SH; Gareiss PC
Journal
Biochemistry, Vol. 48, No. 26, pp. 6146–6156
Publisher
American Chemical Society (ACS)
Publication Date
July 7, 2009
DOI
10.1021/bi900663h
ISSN
0006-2960