Journal article
Cloning of HSP70 (dnaK) gene from Clostridium perfringens using a general polymerase chain reaction based approach
Abstract
A general method to clone the HSP70 gene from any species employing polymerase chain reaction and degenerate oligonucleotide primers for conserved regions of this protein family is described. Using this method, a clone containing the entire coding sequence for the HSP70 gene from Clostridium perfringens, has been isolated and sequenced. The HSP70s from C. perfringens as well as other Gram-positive groups of bacteria contain a large deletion of …
Authors
Galley KA; Singh B; Gupta RS
Journal
Biochimica et Biophysica Acta, Vol. 1130, No. 2, pp. 203–208
Publisher
Elsevier
Publication Date
March 1992
DOI
10.1016/0167-4781(92)90529-9
ISSN
0006-3002