Journal article
Synergy in Induction of Increased Renal Allograft Survival after Portal Vein Infusion of Dendritic Cells Transduced to Express TGFβ and IL-10, along with Administration of CHO Cells Expressing the Regulatory Molecule OX-2
Abstract
Dendritic cells (DC), generated from C57BL/6 mouse bone marrow cells cultured with GM-CSF and IL-4 for 9 days, were engineered to express constitutively the cytokines TGFbeta, IL-10, and IL-12, using adenovirus vectors constructed using an E1-deleted replication-deficient recombinant adenovirus carrying the appropriate cDNA for the relevant cytokines (Ad-TGFbeta, Ad-IL-12, or Ad-IL-10). C3H mice receiving nontransduced DC or pretransplant …
Authors
Gorczynski RM; Bransom J; Cattral M; Huang X; Lei J; Xiaorong L; Min WP; Wan Y; Gauldie J
Journal
Clinical Immunology, Vol. 95, No. 3, pp. 182–189
Publisher
Elsevier
Publication Date
June 2000
DOI
10.1006/clim.2000.4860
ISSN
1521-6616
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AdenoviridaeAdjuvants, ImmunologicAdoptive TransferAnimalsAntigens, CDAntigens, SurfaceCHO CellsCricetinaeCytokinesDendritic CellsFemaleGene Expression RegulationGenetic VectorsGraft SurvivalImmunizationInterleukin-10Kidney TransplantationMaleMiceMice, Inbred BALB CMice, Inbred C3HMice, Inbred C57BLPhenotypePortal VeinSignal TransductionSpleenTransforming Growth Factor beta