On the Interaction of Rabbit Antithrombin III With the Luminal Surface of the Normal and Deendothelialized Rabbit Thoracic Aorta In Vitro

Abstract Pure rabbit antithrombin III was isotope labeled (with 125I or 3H) by two different methods; neither procedure caused a loss of antithrombin activity although both methods affected the affinity of the protein for Sepharose-heparin. From segments from freshly excised rabbit aorta, the uptake of isotope-labeled antithrombin III by the endothelium was rapid and saturable, although relatively small compared to the uptake …