Immune thrombocytopenia (ITP) is an autoimmune bleeding disorder caused by increased platelet destruction and impaired platelet production. Antibody binding to megakaryocytes may occur in ITP, but
in vivoevidence of this phenomenon is lacking. Methods
We determined the proportion of megakaryocytes bound with immunoglobulin G (IgG) in bone marrow samples from primary patients with ITP (
n= 17), normal controls ( n= 13) and thrombocytopenic patients with myelodysplastic syndrome (MDS; n= 10). Serial histological sections from archived bone marrow biopsies were stained for CD61 and IgG. IgG binding and the number of bone marrow megakaryocytes were determined morphologically by a hematopathologist with four assessors after a calibration exercise to ensure consistency. Results
The proportion of ITP patients with high IgG binding (>50% of bone marrow megakaryocytes) was increased compared with normal controls [12/17 (71%) vs. 3/13 (23%),
P= 0.03]. However, the proportion of ITP patients with high IgG binding was no different than thrombocytopenic patients with MDS [12/17 (71%) vs. 7/10 (70%), P= 1.00]. IgG binding was associated with increased megakaryocyte numbers. Like platelet‐associated IgG, megakaryocyte‐associated IgG is related to thrombocytopenia but may not be specific for ITP. Conclusion
Mechanistic studies in ITP should focus on antibody specificity and include thrombocytopenic control patients.