Conference
Detection of methicillin-resistant Staphylococcus aureus (MRSA) from growth on mannitol salt oxacillin agar using PCR for nosocomial surveillance
Abstract
This study evaluated a polymerase chain reaction (PCR) method for detection of methicillin-resistant Staphylococcus aureus (MRSA) in specimens referred for nosocomial surveillance. PCR was used to detect the mecA and nuc gene targets using yellow growth on mannitol salt agar containing 6 mg/liter oxacillin (MSO-6) as a source of DNA (N = 645). The diagnostic values for PCR compared with culture methods were 97% specificity, 100% sensitivity, …
Authors
Jayaratne P; Rutherford C
Volume
35
Pagination
pp. 13-18
Publisher
Elsevier
Publication Date
September 1999
DOI
10.1016/s0732-8893(99)00060-7
Conference proceedings
Diagnostic Microbiology and Infectious Disease
Issue
1
ISSN
0732-8893
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AgarBacterial ProteinsBacteriological TechniquesCarrier ProteinsCross InfectionCulture MediaEndonucleasesHexosyltransferasesHumansMannitolMethicillin ResistanceMicrococcal NucleaseMuramoylpentapeptide CarboxypeptidaseOxacillinPenicillin-Binding ProteinsPenicillinsPeptidyl TransferasesPolymerase Chain ReactionSensitivity and SpecificityStaphylococcal InfectionsStaphylococcus aureus