Journal article
Selective Inhibition of Inward Rectifier K+ Channels (Kir2.1 or Kir2.2) Abolishes Protection by Ischemic Preconditioning in Rabbit Ventricular Cardiomyocytes
Abstract
Volume regulatory Cl- channels are key regulators of ischemic preconditioning (IPC). Because Cl- efflux must be balanced by an efflux of cations to maintain cell membrane electroneutrality during volume regulation, we hypothesize that I(K1) channels may play a role in IPC. We subjected cultured cardiomyocytes to 60-minute simulated ischemia (SI) followed by 60-minute of simulated reperfusion (SR) and assessed percent cell death using trypan …
Authors
Diaz RJ; Zobel C; Cho HC; Batthish M; Hinek A; Backx PH; Wilson GJ
Journal
Circulation Research, Vol. 95, No. 3, pp. 325–332
Publisher
Wolters Kluwer
Publication Date
August 6, 2004
DOI
10.1161/01.res.0000137727.34938.35
ISSN
0009-7330
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AdenoviridaeAnimalsBariumCalcium Channel BlockersCalcium Channels, L-TypeCell SurvivalCells, CulturedChloridesGenetic VectorsIon Channel GatingIon TransportIschemic PreconditioningMyocardial ReperfusionMyocytes, CardiacNifedipinePatch-Clamp TechniquesPotassiumPotassium Channels, Inwardly RectifyingRabbitsRecombinant Fusion ProteinsSarcolemmaTransfection