NOD1 promotes leukocyte clearance and limits inflammation in female mice during obesity-associated acute lung injury. Journal Articles uri icon

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abstract

  • Obesity is associated with metabolic inflammation, which includes changes to innate immune responses relevant to acute lung injury. NOD1 is a cytosolic pattern recognition receptor involved in sensing bacterial peptidoglycan and has been linked to metabolic inflammation. However, its role in obesity-associated acute lung injury, especially in females, remains unclear. Here, we investigated the impact of NOD1 deficiency on pulmonary inflammation in female mice subjected to a high-fat diet and lipopolysaccharide-induced acute lung injury. Compared to wild-type controls, obese Nod1-/- mice showed reduced leukocyte and neutrophil numbers in the bronchoalveolar lavage (BAL), but increased BAL levels of TNF-α, IL-1β, IL-6, IL-17A, and IL-22, suggesting impaired neutrophil clearance. In the lung tissue, NOD1 deficiency during obesity led to elevated neutrophil accumulation, increased myeloperoxidase activity, reduced CD163+ macrophages, and enhanced β-galactosidase activity. Gene expression analysis revealed upregulation of chemokines, adhesion molecules, and inflammasome components, alongside downregulation of M2 polarization markers. Additionally, obese Nod1-/- mice showed higher NF-κB and ERK1/2 activation and lower p38 phosphorylation. These findings indicate that NOD1 regulates leukocyte dynamics, inflammation, and macrophage function in the obese lung. We identify NOD1 as a key protective modulator of pulmonary immune responses during acute lung injury under metabolic stress.

authors

  • Rodrigues E Lacerda, Rodrigo
  • Barra, Nicole G
  • Fang, Han
  • Anhê, Gabriel Forato
  • Schertzer, Jonathan

publication date

  • July 2025