Journal article
Transition-State Analysis of the DNA Repair Enzyme MutY
Abstract
The transition state (TS) structure of MutY-catalyzed DNA hydrolysis was solved using multiple kinetic isotope effect (KIE) measurements. MutY is a base excision repair enzyme which cleaves adenine from 8-oxo-G:A mismatches in vivo, and also from G:A mismatches in vitro. TS analysis of G:A-DNA hydrolysis revealed a stepwise S(N)1 (D(N)*A(N)(double dagger)) mechanism proceeding through a highly reactive oxacarbenium ion intermediate which would …
Authors
McCann JAB; Berti PJ
Journal
Journal of the American Chemical Society, Vol. 130, No. 17, pp. 5789–5797
Publisher
American Chemical Society (ACS)
Publication Date
April 1, 2008
DOI
10.1021/ja711363s
ISSN
0002-7863