Validation of a refined short-term adult fish reproductive test with improved power for mummichog (Fundulus heteroclitus) to test complex effluents

Short-term adult fish reproductive tests are widely used to assess the toxicity of chemicals and waste streams. However, these tests often have low power to detect differences in egg production among treatments, due to high variance and small sample sizes, limiting their effectiveness for informing regulatory decisions. A protocol for a fish reproductive test using mummichog (Fundulus heteroclitus) was refined to increase statistical power. …