Journal article
A ketoreductase domain in the PksJ protein of the bacillaene assembly line carries out both α- and β-ketone reduction during chain growth
Abstract
The polyketide signaling metabolites bacillaene and dihydrobacillaene are biosynthesized in Bacillus subtilis on an enzymatic assembly line with both nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) modules acting along with catalytic domains servicing the assembly line in trans. These signaling metabolites possess the unusual starter unit alpha-hydroxyisocaproate (alpha-HIC). We show here that it arises from initial …
Authors
Calderone CT; Bumpus SB; Kelleher NL; Walsh CT; Magarvey NA
Journal
Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 35, pp. 12809–12814
Publisher
Proceedings of the National Academy of Sciences
Publication Date
September 2, 2008
DOI
10.1073/pnas.0806305105
ISSN
0027-8424