The development and application of a molecular community profiling strategy to identify polymicrobial bacterial DNA in the whole blood of septic patients

BackgroundThe application of molecular based diagnostics in sepsis has had limited success to date. Molecular community profiling methods have indicated that polymicrobial infections are more common than suggested by standard clinical culture. A molecular profiling approach was developed to investigate the propensity for polymicrobial infections in patients predicted to have bacterial sepsis.ResultsDisruption of blood cells with saponin and …