Journal article
Homoserine dehydrogenase from Saccharomyces cerevisiae: kinetic mechanism and stereochemistry of hydride transfer
Abstract
Homoserine dehydrogenase (HSD), which is required for the synthesis of threonine, isoleucine and methionine in fungi, is a potential target for novel antifungal drugs. In order to design effective inhibitors, the kinetic mechanism of Saccharomyces cerevisiae HSD and the stereochemistry of hydride transfer were examined. Product inhibition experiments revealed that yeast HSD follows an ordered Bi Bi kinetic mechanism, where NAD(P)H must bind the …
Authors
Jacques SL; Ejim LJ; Wright GD
Journal
Biochimica Et Biophysica Acta, Vol. 1544, No. 1-2, pp. 42–54
Publication Date
January 12, 2001
ISSN
0006-3002