Conference
Quantification of the Soluble Leptin Receptor in Human Blood by Ligand-Mediated Immunofunctional Assay
Abstract
Panels of monoclonal antibodies (mAbs) were raised against recombinant human leptin and the recombinant human soluble leptin receptor. Using these mAbs, we established a ligand-mediated immunofunctional assay (LIFA) to quantify concentrations of the soluble leptin receptor, which has been shown to be a major binding protein for leptin in human serum. In performing the assay, a monoclonal antibody (mAb 2H6) against the soluble leptin receptor, …
Authors
Wu Z; Bidlingmaier M; Liu C; De Souza EB; Tschöp M; Morrison KM; Strasburger CJ
Volume
87
Pagination
pp. 2931-2939
Publisher
The Endocrine Society
Publication Date
June 2002
DOI
10.1210/jcem.87.6.8610
Conference proceedings
The Journal of Clinical Endocrinology & Metabolism
Issue
6
ISSN
0021-972X