Journal article
RNAi screen identifies Jarid1b as a major regulator of mouse HSC activity
Abstract
Histone methylation is a dynamic and reversible process proposed to directly impact on stem cell fate. The Jumonji (JmjC) domain-containing family of demethylases comprises 27 members that target mono-, di-, and trimethylated lysine residues of histone (or nonhistone) proteins. To evaluate their role in regulation of hematopoietic stem cell (HSC) behavior, we performed an in vivo RNAi-based functional screen and demonstrated that Jarid1b and …
Authors
Cellot S; Hope KJ; Chagraoui J; Sauvageau M; Deneault É; MacRae T; Mayotte N; Wilhelm BT; Landry JR; Ting SB
Journal
Blood, Vol. 122, No. 9, pp. 1545–1555
Publisher
American Society of Hematology
Publication Date
August 29, 2013
DOI
10.1182/blood-2013-04-496281
ISSN
0006-4971
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AnimalsCells, CulturedDNA-Binding ProteinsGene Expression RegulationGene Knockdown TechniquesHematopoiesisHematopoietic Stem CellsHigh-Throughput Screening AssaysHistone DemethylasesJumonji Domain-Containing Histone DemethylasesMiceMice, CongenicMice, Inbred C57BLRNA InterferenceRNA, Small InterferingTranscription FactorsValidation Studies as Topic