Membrane bioreactor separator system for integrated IgG fragmentation and Fab purification

This paper discusses a membrane based bioreactor system for producing pure Fab from human IgG. The bioreactor consisted of a stack of microporous anion-exchange membrane discs housed in a temperature controlled module. IgG was adsorbed on the membrane followed by its fragmentation with papain under optimized conditions. Fab was recovered in the reaction flow through while other fragments remained membrane bound and were subsequently eluted …