Journal article
Probing the Transition States of Four Glucoside Hydrolyses with 13C Kinetic Isotope Effects Measured at Natural Abundance by NMR Spectroscopy
Abstract
Kinetic isotope effects (KIEs) were measured for methyl glucoside (4) hydrolysis on unlabeled material by NMR. Twenty-eight (13)C KIEs were measured on the acid-catalyzed hydrolysis of alpha-4 and beta-4, as well as enzymatic hydrolyses with yeast alpha-glucosidase and almond beta-glucosidase. The 1-(13)C KIEs on the acid-catalyzed reactions of alpha-4 and beta-4, 1.007(2) and 1.010(6), respectively, were in excellent agreement with the …
Authors
Lee JK; Bain AD; Berti PJ
Journal
Journal of the American Chemical Society, Vol. 126, No. 12, pp. 3769–3776
Publisher
American Chemical Society (ACS)
Publication Date
March 1, 2004
DOI
10.1021/ja0394028
ISSN
0002-7863