Journal article
A novel method to map transcripts: Evidence for homology between an adenovirus mRNA and discrete multiple regions of the viral genome
Abstract
A method has been devised which permits mapping of transcripts by a two-step hybridization procedure (sandwich hybridization). RNA extracted from cells infected with an adenovirus-SV40 hybrid (Ad2+ND1) was hybridized to restriction endonuclease fragments of adenovirus type 2 (Ad2) DNA immobilized on nitrocellulose filters. RNAs containing both Ad2 and SV40 sequences formed duplexes through their Ad2 sequences, leaving their SV40 sequences as …
Authors
Dunn AR; Hassell JA
Journal
Cell, Vol. 12, No. 1, pp. 23–36
Publisher
Elsevier
Publication Date
September 1977
DOI
10.1016/0092-8674(77)90182-9
ISSN
0092-8674