Journal article
Mapping Polypeptide Self-Recognition through 1H Off-Resonance Relaxation
Abstract
1H NMR relaxation rates provide a readily available and sensitive probe ideally suited to investigate the weak (KD approximately micromolar to millimolar range) interactions that frequently mediate polypeptide oligomerization in the early steps of amyloid fibrillogenesis. However, the measurement of transverse and longitudinal 1H relaxation rates is experimentally challenging due to J-transfer and selectivity problems in CPMG and …
Authors
Esposito V; Das R; Melacini G
Journal
Journal of the American Chemical Society, Vol. 127, No. 26, pp. 9358–9359
Publisher
American Chemical Society (ACS)
Publication Date
July 1, 2005
DOI
10.1021/ja051714i
ISSN
0002-7863