Lipid Diffusion in Supported Lipid Bilayers: A Comparisonbetween Line-Scanning Fluorescence Correlation Spectroscopyand Single-Particle Tracking

Diffusion in lipid membranes is an essential component of many cellular process and fluorescence a method of choice to study membrane dynamics. The goal of this work was to directly compare two common fluorescence methods, line-scanning fluorescence correlation spectroscopy and single-particle tracking, to observe the diffusion of a fluorescent lipophilic dye, DiD, in a complex five-component mitochondria-like solid-supported lipid bilayer. We …