Journal article
Application of ion-exchange and lipohilic-gel chromatography to the purification and group fractionation of steroidal spirolactones, isolated from biological fluids
Abstract
Chromatography of steroidal spirolactones on DEAE-Sephadex A-25 under selected pH conditions allowed efficient separation of these compounds from other steroids and many of the endogenous components of human urine. The spirolactones were recovered in high yield, mostly over 90%. Lipophilic-gel chromatography provided a useful method for group fractionation of mixtures of these spirolactones with high recoveries (generally over 90%), unaffected …
Authors
Boreham DR; Vose CW; Palmer RF; Brooks CJW; Balasubramanian V
Journal
Journal of chromatography, Vol. 153, No. 1, pp. 63–75
Publisher
Elsevier
Publication Date
June 1978
DOI
10.1016/s0021-9673(00)89856-8
ISSN
0021-9673